HuagongsheHuagongshe Chemistry Print Report · For research reference only
Borate → Biotin
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Borate | HCID 26574 | — |
| Reactant | Biotin-X-NHS | HCID 5063989 | — |
| Solvent | Sodium Chloride | HCID 5234 | — |
| Product | Biotin | HCID 171548 | — |
Reaction Steps
50 mg bovine serum albumin (BSA) (Fraction V, Fluka) were dissolved in pH 8.5 50 mM NaCl, 20 mM borate buffer. A solution of biotin X NHS (20 mg/200 μl anhydrous dimethylformamide) was then added, and the following steps were identical to those described for the preparation of biotinylated XO.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05108893 |
| Source dataset | uspto-grants-1992_04 |
| ORD ID | ord-e65b83099e77453692e7ad7e7d2dd796 |
| Source note | uspto-grants-1992_04 |