Water → Sorbitol

In the same manner as described in Example 3, the seed culture (200 ml) of the mixture of Gluconobacter suboxydans IFO 3255 and the strain DSM No. 4025 was inoculated into 3-L fermenter containing 8% D-sorbitol in the fermentation medium and the working volume was adjusted to 2 L with sterilized water. The fermentation was started under the same conditions as described in Example 3. Separately, a 500 ml medium bottle charged with 300 ml of feeding medium: 120 g of D-sorbitol, 10 g of yeast extract, 50 g of corn steep liquor, 0.172 g of KH2PO4, 1.72 g of urea (sterilized separately) and 2.5 g of antifoam, was prepared. During the fermentation, the above feeding medium was continuously fed to the fermenter at the rate of 15 ml/hr between the fermentation period of 12 and 18 hours, 60 ml/hr between that of 18 hours and 21 hours and 4.3 ml/hr between that of 21 hours and 28 hours by using a peristaltic pump. As a result, 224 g of 2-keto-L-gulonic acid was produced from 276 g of D-sorbitol at the conversion yield of 76.1 mol % in 51 hours fermentation.

1. additionSeparately, a 500 ml medium bottle charged with 300 ml
2. extraction120 g of D-sorbitol, 10 g of yeast extract
3. custom50 g of corn steep liquor, 0.172 g of KH2PO4, 1.72 g of urea (sterilized separately) and 2.5 g of antifoam, was prepared
4. customof 18 hours
5. custom28 hours

ORD record

ord-a6657c5d891948b491ad1460dac62583

Source dataset

uspto-grants-1994_05

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05312741