Glycerol → Sucrose

HRID 1033726Notes

Reaction equation

[Cl-].[K+].[OH:3][CH2:4][CH:5]([CH2:7][OH:8])[OH:6]>>[CH2:4]([OH:3])[C@H:5]1[O:6][C@H:7]([O:6][C@:5]2([CH2:7][OH:8])[O:6][C@H:5]([CH2:7][OH:8])[C@@H:4]([OH:3])[C@@H:4]2[OH:3])[C@H:5]([OH:6])[C@@H:4]([OH:3])[C@@H:7]1[OH:8]

Participants

Reactant 2

HCID 753Glycerol

HCID 4873Potassium Chloride2×

Product 1

HCID 5988Sucrose

Procedure

Sucrose density gradients (8-30%) were prepared in TT buffer containing 0.4 M KCl. In some experiments (as indicated) the gradients were made 8-30% in TT buffer containing 10% glycerol with or without 0.4 M KCl. Samples to be analyzed were layered on the gradient together with 14C-labelled sedimentation markers. The gradients were centrifuged at 50,000 rpm in an SW60 rotor for 18 h at 2° C. Gradients were fractionated into individual 0.1 ml fractions, scintillation fluid was added, and radioactivity counted.

Workup

additionwas added

Sources & evidence

ORD record: ord-0dd44a4ce64644d197b32a26152f9510
Source dataset: uspto-grants-1994_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05312752

Reaction notes

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