反应详情

Tert-butyl 2-methyl-1-((R)-1-((4S,5R)-2-methyl-1-oxo-4-phenyl-2,7-diazaspiro[4.5]decan-7-yl)-1-oxo-4-phenoxybutan-2-ylamino)-1-oxopropan-2-ylcarbamate (38.7 mg; 0.064 mmol) was solubilised in DCM (0.5 ml). TFA (0.125 ml; 1.622 mmol) was added and the solution stirred at RT for 20 minutes before being concentrated in vacuo. The residual oil was dissolved in methanol (1 ml) and passed through a 1 g SCX-2 cartridge eluting with 2M NH3 in methanol (4 ml. Evaporation gave the title compound.

1. concentrationbefore being concentrated in vacuo
2. dissolutionThe residual oil was dissolved in methanol (1 ml)
3. washeluting with 2M NH3 in methanol (4 ml
4. customEvaporation