反应详情

A solution was prepared by the addition of 1 mmol of EDC and 0.2 mmol of NHS to 1 ml of DMSO, and 50 μl of the thus prepared solution was added onto the CMD film. The solution was then allowed to undergo the reaction at the room temperature for 30 minutes. After the solution was removed, washing with DMSO was performed one time. Also, a solution prepared by the addition of 0.1 mmol of AB-NTA (supplied by Dojin Kagaku K.K.) to 1 ml of DMSO was allowed to undergo the reaction for 12 hours. The solution was removed, and washing with ultra pure water was performed one time.

1. additionwas added onto the CMD film
2. customthe reaction at the room temperature for 30 minutes
3. customAfter the solution was removed
4. washwashing with DMSO
5. customthe reaction for 12 hours
6. customThe solution was removed
7. washwashing with ultra pure water