反应详情

温度

120 °C

4-Chloro-7-(3,5-dimethyl-4-isoxazolyl)-6-(methyloxy)-3-quinolinecarboxamide (for a preparation see Intermediate 56, 50 g, 151 mmol,) and [(1R)-1-(2-pyridinyl)ethyl]amine (35.3 g, 181 mmol, NetChem) were dissolved in N-methyl-2-pyrrolidone (NMP, 250 ml). DIPEA (79 ml, 452 mmol) was added, the solution was heated at 120° C. overnight, cooled and diluted with ethyl acetate (1 l). The solution was washed with water (2×1 l), brine (500 ml), dried (sodium sulphate) and the solvent evaporated to give a dark brown gum. The aqueous washings were extracted with DCM (2×600 ml). The combined extracts were washed with a mixture of saturated brine (300 ml) and water (1 l) giving a dense emulsion which took around 2 h to separate. The organic layer was dried (sodium sulphate) and evaporated to leave a brown liquid. This liquid was dissolved in ethyl acetate (200 ml), washed with water (2×200 ml), dried (sodium sulphate) and evaporated to give a brown gum. This material was combined with the previous brown gum, dissolved in DCM (150 ml) and loaded onto a silica column (750 g), which was eluted with a 2M ammonia in methanol/DCM gradient (0-12%) to give, after evaporation of the solvents in vacuo 7-(3,5-dimethyl-4-isoxazolyl)-6-(methyloxy)-4-{[(1R)-1-(2-pyridinyl)ethyl]amino}-3-quinolinecarboxamide as beige foam (45.1 g). Used in the subsequent step (Example 36) without further purification.

1. temperaturecooled
2. washThe solution was washed with water (2×1 l), brine (500 ml)
3. dry with materialdried (sodium sulphate)
4. customthe solvent evaporated
5. customto give a dark brown gum
6. extractionThe aqueous washings were extracted with DCM (2×600 ml)
7. washThe combined extracts were washed with a mixture of saturated brine (300 ml) and water (1 l)
8. customgiving a dense emulsion which
9. customto separate
10. dry with materialThe organic layer was dried (sodium sulphate)
11. customevaporated
12. customto leave a brown liquid
13. washwashed with water (2×200 ml)
14. dry with materialdried (sodium sulphate)
15. customevaporated
16. customto give a brown gum
17. washwas eluted with a 2M ammonia in methanol/DCM gradient (0-12%)
18. customto give
19. customafter evaporation of the solvents in vacuo 7-(3,5-dimethyl-4-isoxazolyl)-6-(methyloxy)-4-{[(1R)-1-(2-pyridinyl)ethyl]amino}-3-quinolinecarboxamide as beige foam (45.1 g)
20. customUsed in the subsequent step (Example 36) without further purification