反应详情

Add hydrogen chloride (1N, 0.53 mL; 1.0 equiv; 0.53 mmoles) to a solution of 5,6,7,8-tetrahydroimidazo[1,2-a]pyridin-6-ylmethyl 2-(2,3-dihydro-1H-inden-2-ylamino)-5,7-dihydro-6H-pyrrolo[3,4-d]pyrimidine-6-carboxylate isomer 1 (0.228 g; 1.0 equiv; 0.53 mmoles) in methanol (0.5 mL). Swirl the mixture until dissolution occurs and then concentrate to a residue. Add water (1 mL), freeze the solution in a −78° C. dry ice bath, and lyophilize to afford 5,6,7,8-tetrahydroimidazo[1,2-a]pyridin-6-ylmethyl 2-(2,3-dihydro-1H-inden-2-ylamino)-5,7-dihydro-6H-pyrrolo[3,4-d]pyrimidine-6-carboxylate hydrochloride isomer 1 (0.238 g; 100%) as a tan powder. MS (m/z): 431(M+1).

1. dissolutionSwirl the mixture until dissolution
2. concentrationconcentrate to a residue
3. customin a −78° C.