Sodium Chloride → HCID 178418600, compound

Participants

Reaction Steps

The flask culture of each of argG promoter modified strains was conducted. As controls, parent strain AJ12092 and AJ12092/pargG were also cultured. These strains were each inoculated into a medium [containing 0.1 g/dl of KH2PO4, 0.04 g/dl of MgSO4, 0.001 g/dl of FeSO4, 0.01 g/dl of MnSO4, 5 μg/dl of VB1, 5 μg/dl of biotin and 45 mg/dl (in terms of N) of soybean hydrolyzate]; and then spread on an agar medium (containing 0.5 g/dl of glucose, 1 g/dl of polypeptone, 1 g/dl of yeast extract, 0.5 g/dl of NaCl and 5 μg/l of chloramphenicol), and cultured at 31.5° C. for 20 hours. One aze of the cells were cultured in a flask containing 4 g/dl of glucose and 6.5 g/dl of ammonium sulfate at 31.5° C. until glucose had been completely consumed. The absorbance (CD620) of the culture liquid diluted to a concentration of 1/51 with 0.2 N HCl solution, the quantity of arginine produced (concentration: g/dl) and culture time were shown in Table 22.

Provenance & Evidence