Sodium Chloride → HCID 178418600, compound

HRID 1062513Notes

Reaction equation

HRID 1062513 reaction equation
OP([O-])(O)=O.[K+].[O-]S([O-])(=O)=O.[Mg+2].OC(CCCC[C@H]1[C@@H]2[C@@H]([NH:24][C:25]([NH:27]2)=O)CS1)=O.O=C[C@@H:31]([C@H:33]([C@@H:35]([C@@H:37]([CH2:39][OH:40])O)O)O)O.[Na+].[Cl-].C1C([C@@H](O)[C@H](NC(C(Cl)Cl)=O)C[OH:52])=CC=C([N+]([O-])=O)C=1.S([O-])([O-])(=O)=O.[NH4+:68].[NH4+:69]>[O-]S([O-])(=O)=O.[Mn+2]>[NH2:68][C@H:37]([C:39]([OH:40])=[O:52])[CH2:35][CH2:33][CH2:31][NH:69][C:25](=[NH:24])[NH2:27]

Participants

9

Reactant 7

HCID 5234Sodium ChlorideHCID 5959ChloramphenicolHCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal3×HCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

Product 1

HCID 178418600HCID 178418600, compound

Reagents, catalysts & solvents 1

HCID 24580Manganese SulfateCatalyst

Procedure

The flask culture of each of argG promoter modified strains was conducted. As controls, parent strain AJ12092 and AJ12092/pargG were also cultured. These strains were each inoculated into a medium [containing 0.1 g/dl of KH2PO4, 0.04 g/dl of MgSO4, 0.001 g/dl of FeSO4, 0.01 g/dl of MnSO4, 5 μg/dl of VB1, 5 μg/dl of biotin and 45 mg/dl (in terms of N) of soybean hydrolyzate]; and then spread on an agar medium (containing 0.5 g/dl of glucose, 1 g/dl of polypeptone, 1 g/dl of yeast extract, 0.5 g/dl of NaCl and 5 μg/l of chloramphenicol), and cultured at 31.5° C. for 20 hours. One aze of the cells were cultured in a flask containing 4 g/dl of glucose and 6.5 g/dl of ammonium sulfate at 31.5° C. until glucose had been completely consumed. The absorbance (CD620) of the culture liquid diluted to a concentration of 1/51 with 0.2 N HCl solution, the quantity of arginine produced (concentration: g/dl) and culture time were shown in Table 22.

Workup

  1. customOne aze of the cells were cultured in a flask
  2. customhad been completely consumed
  3. additionThe absorbance (CD620) of the culture liquid diluted to a concentration of 1/51 with 0.2 N HCl solution

Sources & evidence

ORD record
ord-3c12fb76074a400d900fb12946afe0ed
Source dataset
uspto-grants-2005_11
Patent number
US06962805B2

Reaction notes

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