tert-Butyl methyl ether → HCID 9793812, compound

Assay of plasma samples: to test tubes containing 2 μg of the internal standard (diisopropyl acetamide), 0.5 ml of plasma (thawed at room temperature) and 5 ml of tert-butyl methyl ether (TBME) was added. The test tubes were vigorously vortexed for 30 sec and centrifuged at 3000 g for 10 minutes. The organic phase was separated and dried under reduced pressure using a vortex evaporator. Samples were then reconstituted with 150 μl of chloroform and dried under reduced pressure without vortexing. The sample was again reconstituted with 30 μl of chloroform, of which 2 μl were injected into the GC apparatus.

1. additionwas added
2. waitcentrifuged at 3000 g for 10 minutes
3. customThe organic phase was separated
4. customdried under reduced pressure
5. customa vortex evaporator
6. customdried under reduced pressure without vortexing

ORD record

ord-19039cf30e564ed1a92aacb0a5922940

Source dataset

uspto-grants-2005_11

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06969732B2