反应详情

A suspension of 10 mg/ml of an adenosine deaminase enzyme (prepared from intestinal calf mucosa) was assayed to have an activity of 210 μ moles/min. at 38° C. To a solution of 191 mg. of 2,6-diamino-9-(β-D-arabinofuranosyl)purine in 800 ml of a 1 millimole phosphate buffer of pH 7.1 was added 50 micrograms of adenosine deaminase. After two hours at 38° C. the reaction mixture was freeze dried. The resulting powder was extracted with hot methanol which was filtered and evaporated to give a solid. This solid was triturated with methanol, filtered and the methanol evaporated. The solid was dissolved in 25 ml. of warm methanol and cooled to crystallize the product, 2-amino-6-hydroxy-9-(β-D-arabinofuranosyl)purine, m.p. < 300° C.

1. customat 38° C
2. customfreeze dried
3. extractionThe resulting powder was extracted with hot methanol which
4. filtrationwas filtered
5. customevaporated
6. customto give a solid
7. customThis solid was triturated with methanol
8. filtrationfiltered
9. customthe methanol evaporated
10. dissolutionThe solid was dissolved in 25 ml
11. temperatureof warm methanol and cooled
12. customto crystallize the product, 2-amino-6-hydroxy-9-(β-D-arabinofuranosyl)purine, m.p. < 300° C.