Phosphate → HCID 178508927, compound

Participants

Reaction Steps

A suspension of 10 mg/ml of an adenosine deaminase enzyme (prepared from intestinal calf mucosa) was assayed to have an activity of 210 μ moles/min. at 38° C. To a solution of 191 mg. of 2,6-diamino-9-(β-D-arabinofuranosyl)purine in 800 ml of a 1 millimole phosphate buffer of pH 7.1 was added 50 micrograms of adenosine deaminase. After two hours at 38° C. the reaction mixture was freeze dried. The resulting powder was extracted with hot methanol which was filtered and evaporated to give a solid. This solid was triturated with methanol, filtered and the methanol evaporated. The solid was dissolved in 25 ml. of warm methanol and cooled to crystallize the product, 2-amino-6-hydroxy-9-(β-D-arabinofuranosyl)purine, m.p. < 300° C.

Provenance & Evidence