Water → Theophylline

HRID 1078176Notes

Reaction equation

HRID 1078176 reaction equation
C([C:4]1[NH:16][C:15]2[C:14](=[O:17])[N:9]([CH2:10]C(O)=O)[C:8](=[O:18])[N:7]([CH3:19])[C:6]=2[N:5]=1)CC.ON1C(=O)CCC1=O.O>CN(C=O)C>[N:9]1([C:14](=[O:17])[C:15]2[NH:16][CH:4]=[N:5][C:6]=2[N:7]([CH3:19])[C:8]1=[O:18])[CH3:10]

Participants

5

Reactant 3

HCID 962WaterHCID 80170N-HydroxysuccinimideHCID 86745509HCID 86745509, compound

Product 1

HCID 2153Theophylline

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent

Procedure

5 mg of 8-propylcarboxytheophylline was dissolved in 1 ml of DMF, and added with 3 mg of N-hydroxysuccinimide and 5 mg of water-soluble carbodiimide, followed by stirring at room temperature for 2 hours, to obtain activated theophylline. 10 mg of bovine serum albumin (BSA) was dissolved in 1 ml of a 0.1M aqueous solution of sodium hydrogencarbonate and added with 500 μl of the aforementioned activated theophylline solution. The mixed solution was allowed to stand at room teperature for an hour, and then unreacted substances were removed through a Sephadex-G25 column preliminarily equilibrated with PBS to obtain 9 mg of the objective large molecule antigen (theophylline-BSA conjugate)

Sources & evidence

ORD record
ord-ab924a6d40404d9d92c3bce2666e2adc
Source dataset
uspto-grants-1996_08
Patent number
US05547848

Reaction notes

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