Alpha-Aminoadipic Acid → D-Glutamic Acid

Participants

Reaction Steps

To measure the D-aminoacid transaminase activity, D-α-aminoadipic acid (20 mmolar), α-ketoglutarate (10 mmolar) and pyridoxal phosphate (10 μg/ml) are incubated with the enzyme in potassium phosphate buffer (10 mmolar), pH 8, at a temperature of 37° C. This results in the formation of D-glutamic acid which can be determined by thin-layer chromatography after spraying with ninhydrin.

Provenance & Evidence