Alpha-Aminoadipic Acid → D-Glutamic Acid

HRID 1081554Notes

Reaction equation

HRID 1081554 reaction equation
C(CC(O)=O)CC([NH2:7])C(O)=O.O=[C:13]([CH2:17][CH2:18][C:19]([O-:21])=[O:20])[C:14]([O-:16])=[O:15].CC1N=CC(COP(O)(O)=O)=C(C=O)C=1O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[NH2:7][C@@H:13]([C:14]([OH:16])=[O:15])[CH2:17][CH2:18][C:19]([OH:21])=[O:20]

Participants

5

Reactant 3

HCID 469Alpha-Aminoadipic AcidHCID 1051Pyridoxal 5'-phosphateHCID 164533Alpha-Ketoglutarate

Product 1

HCID 23327D-Glutamic Acid

Reagents, catalysts & solvents 1

HCID 62657Potassium Phosphate, TribasicSolvent

Procedure

To measure the D-aminoacid transaminase activity, D-α-aminoadipic acid (20 mmolar), α-ketoglutarate (10 mmolar) and pyridoxal phosphate (10 μg/ml) are incubated with the enzyme in potassium phosphate buffer (10 mmolar), pH 8, at a temperature of 37° C. This results in the formation of D-glutamic acid which can be determined by thin-layer chromatography after spraying with ninhydrin.

Workup

  1. customat a temperature of 37° C

Sources & evidence

ORD record
ord-01f584313ced43958c694936b90dbc44
Source dataset
uspto-grants-1996_12
Patent number
US05587319

Reaction notes

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