beta-Maltose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

To measure the enzymatic activity of the nigerooligosaccharide glucanotransferase, 0.25 ml of an enzyme solution is added in 0.75 ml of a maltose solution prepared by dissolving maltose in a 20 mM phosphate buffer (pH 7.0) in a concentration of 0.66% by weight, to carry out reaction at 37° C., where the amount of enzyme for forming 1 μmol of glucose in one minute from the substrate maltose is defined to be one unit.

Provenance & Evidence