beta-Maltose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1106370Notes

Reaction equation

HRID 1106370 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]>P([O-])([O-])([O-])=O>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:23])[OH:7])[OH:22])[OH:21])[OH:20]

Participants

3

Reactant 1

HCID 6255beta-Maltose3×

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent

Procedure

To measure the enzymatic activity of the nigerooligosaccharide glucanotransferase, 0.25 ml of an enzyme solution is added in 0.75 ml of a maltose solution prepared by dissolving maltose in a 20 mM phosphate buffer (pH 7.0) in a concentration of 0.66% by weight, to carry out reaction at 37° C., where the amount of enzyme for forming 1 μmol of glucose in one minute from the substrate maltose is defined to be one unit.

Workup

  1. customprepared
  2. customreaction at 37° C.

Sources & evidence

ORD record
ord-a1166a7b194c47f4bf19a9e62db9daee
Source dataset
uspto-grants-2000_05
Patent number
US06056980

Reaction notes

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