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100 ml of a nutrient medium, containing 1% cornsteep liquor and 1% glucose, were adjusted to pH 6.5 and, after sterilization, inoculated with the mycelium of IFO 6469. After incubation at 26.5° on the rotary shaking machine during 22 hours, 100 mg of 17β-hydroxy-4-androsten-3-one in 1 ml of dimethyl sulphoxide were added and the incubation was continued at 26.5° for a further 8 days. Thereafter, the fermentation solution was filtered, the filtrate was extracted with ethyl acetate and concentrated to a small volume and under reduced pressure. The concentrate was chromatographed on silicic acid with chloroform-acetone. There were obtained 17.9 mg of 7α,17β-dihydroxy-4-androsten-3-one.

1. waitthe incubation was continued at 26.5° for a further 8 days
2. filtrationThereafter, the fermentation solution was filtered
3. extractionthe filtrate was extracted with ethyl acetate
4. concentrationconcentrated to a small volume and under reduced pressure
5. customThe concentrate was chromatographed on silicic acid with chloroform-acetone