Acetic Acid → Ethyl (3-(n-(tert-butoxycarbonyl)amino)-1-fluoro-2-oxopropyl)(1,1-diethoxyethyl)phosphinate

Temperature

-78 °C

To a solution of diisopropylamine (2.5 mL, 14.5 mmol, 3.5 eq) in THF (30 mL) at −10° C. was added dropwise (about 10 minutes) n-BuLi (1.4 M in hexanes, 9.0 mL, 14.5 mmol). After 10 minutes, the reaction was cooled to −78° C., and a solution of ethyl (fluoromethyl)(1,1-diethoxyethyl)phosphinate (2.0 g, 8.26 mmol, 2 eq) in THF (10 mL) was added dropwise over 10 minutes. After the addition the reaction mixture was stirred at −78° C. for 1 h. A solution of N-Boc-glycine methyl ester (0.8 g, 4.1 mmol) in THF (10 mL) was added dropwise over 10 minutes such that the internal temperature was maintained below −70° C. After the addition was complete the reaction was stirred at −78° C. for 1 h. The reaction was quenched with acetic acid (1 mL, 14.5 mmol) and then warmed to room temperature. Saturated aqueous sodium chloride (75 mL) was added to the reaction and the organic phase separated. The aqueous phase was then extracted with ethyl acetate (2×75 mL). The combined organic phase was dried over anhydrous sodium sulfate and concentrated under reduced pressure affording the crude product as a pale yellow oil (2.69 g). The crude product was purified by column chromatography (2×35 cm column), eluting with 40% ethyl acetate in hexane. This method afforded ethyl (3-(N-(tert-butoxycarbonyl) amino)-1-fluoro-2-oxopropyl)(1,1-diethoxyethyl)phosphinate as a clear colorless oil (0.73 g, 44%). 1H NMR (300 MHz, CDCl3) δ 5.78-5.24 (m,2H), 4.52-4.08 (m, 4H), 3.94-3.50 (m, 4H), 1.62-1.51 (m, 3H), 1.50-1.32 (m, 3H), 1.42 (s, 9H), 1.30-1.12 (m, 6H).

1. additionAfter the addition the reaction mixture
2. temperaturewas maintained below −70° C
3. additionAfter the addition
4. stirringwas stirred at −78° C. for 1 h
5. temperaturewarmed to room temperature
6. customthe organic phase separated
7. extractionThe aqueous phase was then extracted with ethyl acetate (2×75 mL)
8. dry with materialThe combined organic phase was dried over anhydrous sodium sulfate
9. concentrationconcentrated under reduced pressure
10. customaffording the crude product as a pale yellow oil (2.69 g)
11. customThe crude product was purified by column chromatography (2×35 cm column)
12. washeluting with 40% ethyl acetate in hexane

ORD record

ord-ee93a9dc3f8648f091d09d5ea89c6374

Source dataset

uspto-grants-2010_10

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07807658B2