反应详情

To a solution of 5-hydroxyanthranilic acid (7.6 g, 49.3 mmol) in DMF (100 mL) was added 1-[3-dimethylaminopropyl]-3-ethylcarbodiimide hydrochloride (EDC) (10.4 g, 54.2 mmol), 1-hydroxybenzotriazole hydrate (HOBt) (7.3 g, 54.2 mmol), 2-amino-N-isopropyl-acetamide hydrochloride (INTERMEDIATE I.1) (7.5 g, 49.3 mmol) and N,N-diisopropylethylamine (DIEA) (9.50 mL, 7.0 g, 54.2 mmol). The reaction mixture was stirred at room temperature for 24 h, concentrated in vacuo to half of its volume and partitioned between diethyl ether (100 mL) and 1N HCl (aq.) (400 mL). The aqueous layer was extracted with diethyl ether (5×100 mL), and ethyl acetate (5×100 mL). The aqueous layer was then concentrated under high vacuum to dryness. The red residue was dissolved in ethanol (20 mL) followed by addition of dichloromethane (1 L). The resulting brown precipitate was filtered under vacuum and washed with a minimum amount of methanol to give the desired product 2-amino-5-hydroxy-N-(isopropylcarbamoylmethyl)benzamide (INTERMEDIATE I.1) hydrochloride salt as a white solid (5.7 g, 19.8 mmol, 40%).

1. concentrationconcentrated in vacuo to half of its volume
2. custompartitioned between diethyl ether (100 mL) and 1N HCl (aq.) (400 mL)
3. extractionThe aqueous layer was extracted with diethyl ether (5×100 mL), and ethyl acetate (5×100 mL)
4. concentrationThe aqueous layer was then concentrated under high vacuum to dryness
5. dissolutionThe red residue was dissolved in ethanol (20 mL)
6. additionfollowed by addition of dichloromethane (1 L)
7. filtrationThe resulting brown precipitate was filtered under vacuum
8. washwashed with a minimum amount of methanol