反应详情

[2-(3-Chloro-propoxy)-phenyl]-(2-chloro-9-cyclopentyl-purin-6-yl)-amine (0.4 mmol) are stirred at RT in N-methylpiperazine (0.3 ml) for 12 hours. The mixture is diluted with water and extracted 3 times with ethyl acetate. The combined organic phases are washed with brine and the extract is dried over sodium sulfate and evaporated. The residue is purified by flash chromatography on silica gel with (CH2Cl2/MeOH=9:1) as mobile phase. The product containing fractions are combined and evaporated. A pale oil with Rf=0.25 (CH2Cl2/MeOH=9:2) is obtained.

1. extractionextracted 3 times with ethyl acetate
2. washThe combined organic phases are washed with brine
3. dry with materialthe extract is dried over sodium sulfate
4. customevaporated
5. customThe residue is purified by flash chromatography on silica gel with (CH2Cl2/MeOH=9:1) as mobile phase
6. additionThe product containing fractions
7. customevaporated
8. customA pale oil with Rf=0.25 (CH2Cl2/MeOH=9:2) is obtained