Sodium Hydroxide → L-Malic Acid

HRID 1165741Notes

Reaction equation

[C:1]([OH:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([OH:6])=[O:5].[OH-:9].[Na+]>>[C:1]([OH:8])(=[O:7])[C@H:2]([CH2:3][C:4]([OH:6])=[O:5])[OH:9]

Participants

Reactant 2

HCID 14798Sodium Hydroxide

HCID 444972Fumaric Acid

Product 1

HCID 222656L-Malic Acid

Procedure

For the purpose of examination as to fumarase activity, the bacterial cells were suspended in 100 ml of a 1.0 M aqueous solution of fumaric acid having a pH adjusted to 7.0 by NaOH and were reacted at 45° C. for 2 hours and the L-malic acid thereby formed was reacted with 2,7-naphthalenediol in the presence of sulfuric acid. The amount of the L-malic acid was determined by the color which it had developed. The amount of the succinic acid which had been formed was determined by high pressure liquid chromatography.

Workup

customwere reacted at 45° C. for 2 hours

Sources & evidence

ORD record: ord-833db5eb14084500ad2138a8422a555c
Source dataset: uspto-grants-1990_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04912043

Reaction notes

Loading…