Sodium Hydroxide → L-Malic Acid

Participants

Reaction Steps

For the purpose of examination as to fumarase activity, the bacterial cells were suspended in 100 ml of a 1.0 M aqueous solution of fumaric acid having a pH adjusted to 7.0 by NaOH and were reacted at 45° C. for 2 hours and the L-malic acid thereby formed was reacted with 2,7-naphthalenediol in the presence of sulfuric acid. The amount of the L-malic acid was determined by the color which it had developed. The amount of the succinic acid which had been formed was determined by high pressure liquid chromatography.

Provenance & Evidence