反应详情

A mixture of 2-[3-(tert-butoxycarbonyl)amino-benzo[d]isoxazol-5-ylmethyl]-isoindole-1,3-dione (1.31 g) and hydrazine hydrate (2.52 mL, 52 mmol) in 1-butanol (10 mL) was stirred at room temperature. The mixture turned into a translucent orange solution. After stirring for about 1 hr, white precipitate formed. The mixture was then heated to reflux for 5 min and all solid dissolved. After cooling, DCM was added, after which time white precipitate formed. The solid was filtered off and washed with DCM. Rotary evaporation afforded a yellow oil. The oily residue was taken up with EtOAc (100 mL) and washed with water (3×30 mL), brine (30 mL) and dried over anhydrous sodium sulfate. Most solvent was removed on a rotary evaporator and the remainder was loaded onto a 20 g ISOLUTE silica cartridge. The product was eluted with (2 M ammonia in methanol)/DCM (4:96). A light yellow solid was obtained (330 mg). 1H-NMR (300 MHz, CDCl3) δ 8.05 (s, 1H), 7.53 (d, 1H), 7.45 (d, 1H), 7.31 (s, 1H), 3.40 (s, 2H), 1.57 (s, 9H).

1. stirringAfter stirring for about 1 hr
2. customwhite precipitate formed
3. temperatureThe mixture was then heated
4. temperatureto reflux for 5 min
5. dissolutionall solid dissolved
6. temperatureAfter cooling
7. customafter which time white precipitate formed
8. filtrationThe solid was filtered off
9. washwashed with DCM
10. customRotary evaporation
11. customafforded a yellow oil
12. washwashed with water (3×30 mL), brine (30 mL)
13. dry with materialdried over anhydrous sodium sulfate
14. customMost solvent was removed on a rotary evaporator
15. washThe product was eluted with (2 M ammonia in methanol)/DCM (4:96)
16. customA light yellow solid was obtained (330 mg)