反应详情

The compound (52.0 mg) obtained in Example 47-3 was dissolved in methanol (1.6 ml) and then added with 1-methylimidazol-2-aldehyde (16.4 mg), and sodium cyanoborohydride (15.6 mg). The solution was adjusted to pH 5 with acetic acid and stirred at room temperature for 14 hours. After completion of the reaction, the reaction solution was added with a 1 mol/l sodium hydroxide aqueous solution and then separated and extracted with chloroform. The organic layer was dried with anhydrous sodium sulfate. The solvent was distilled off and the residue was then purified through silica gel column chromatography (chloroform/methanol) and treated with hydrochloric acid, thereby obtaining hydrochloride (15.2 mg) of the subject compound as a pale-yellow solid.

1. customAfter completion of the reaction
2. customseparated
3. extractionextracted with chloroform
4. dry with materialThe organic layer was dried with anhydrous sodium sulfate
5. distillationThe solvent was distilled off
6. customthe residue was then purified through silica gel column chromatography (chloroform/methanol)
7. additiontreated with hydrochloric acid