反应详情

The compound (54.8 mg) obtained in Example 127-14 was dissolved in methanol (2.74 ml) and added with 1-methyl-2-imidazole carboxaldehyde (21.9 mg) and sodium cyanoborohydride (16.7 mg). Then, the solution was adjusted to pH 4 with acetic acid and stirred at room temperature for 3 hours. The reaction solution was concentrated under reduced pressure and added with saturated saline solution. The solution was subjected to separation/extraction with chloroform. The extract was dried with anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified through silica gel column chromatography (chloroform/methanol) and treated with hydrochloric acid, thereby obtaining hydrochloride (35.9 mg) of the subject compound as a white solid.

1. concentrationThe reaction solution was concentrated under reduced pressure
2. additionadded with saturated saline solution
3. extractionThe solution was subjected to separation/extraction with chloroform
4. dry with materialThe extract was dried with anhydrous sodium sulfate
5. concentrationconcentrated under reduced pressure
6. customThe residue was purified through silica gel column chromatography (chloroform/methanol)
7. additiontreated with hydrochloric acid