3-Cyanopyridine → Nicotinamide

HRID 1174455Notes

Reaction equation

[C:1]([C:3]1[CH:4]=[N:5][CH:6]=[CH:7][CH:8]=1)#[N:2].P([O-])([O-])([O-])=[O:10].[K+].[K+].[K+]>>[C:1]([NH2:2])(=[O:10])[C:3]1[CH:8]=[CH:7][CH:6]=[N:5][CH:4]=1

Participants

Reactant 2

HCID 793-Cyanopyridine

HCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 936Nicotinamide

Conditions

Temperature: 20 °C

Procedure

Nitrile hydratase activity was determined by incubating a reaction mixture comprising 3-cyanopyridine (1.0 M; 1.0 ml), potassium phosphate buffer (0.1 M, pH 7.0; 0.5 ml) and cell suspension (0.5 ml) with stirring at 20° C. for 5 min. The reaction was stopped by adding HCI (5 M; 0.1 ml). After filtration (0.2 μm filter) of the reaction mixture, the amount of nicotinamide produced was determined by means of HPLC (Waters Spherisorb 5 μ ODS2 (4.6×150 mm); KH2P04/H3P04 (10 mM; pH 2.8)/acetonitrile=9:1 (v/v); 1 ml/min; 230 nm). The total activity is expressed as μmol of nicotinamide produced/ (min×ml) and the specific activity is expressed as μmol of nicotinamide produced/(min×ml×OD610 nm).

Workup

additionby adding HCI (5 M; 0.1 ml)
filtrationAfter filtration (0.2 μm filter) of the reaction mixture

Sources & evidence

ORD record: ord-6f63ea54971c47a1838705bbc1211ef0
Source dataset: uspto-grants-2010_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07838271B2

Reaction notes

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