3-Cyanopyridine → Nicotinamide

Participants

Reaction Conditions

Reaction Steps

Nitrile hydratase activity was determined by incubating a reaction mixture comprising 3-cyanopyridine (1.0 M; 1.0 ml), potassium phosphate buffer (0.1 M, pH 7.0; 0.5 ml) and cell suspension (0.5 ml) with stirring at 20° C. for 5 min. The reaction was stopped by adding HCI (5 M; 0.1 ml). After filtration (0.2 μm filter) of the reaction mixture, the amount of nicotinamide produced was determined by means of HPLC (Waters Spherisorb 5 μ ODS2 (4.6×150 mm); KH2P04/H3P04 (10 mM; pH 2.8)/acetonitrile=9:1 (v/v); 1 ml/min; 230 nm). The total activity is expressed as μmol of nicotinamide produced/ (min×ml) and the specific activity is expressed as μmol of nicotinamide produced/(min×ml×OD610 nm).

Provenance & Evidence