反应详情

温度

25 °C

A mixture of 2-methyl-4-(5-(1-phenylcyclopropyl)thiazolo[5,4-b]pyridine-2-yl)benzaldehyde (22.6 mg, 61 μmol), azetidine-3-carboxylic acid (31 mg, 305 μmol), and acetic acid (28 μL, 488 μmol) in 1:1 CH2Cl2/MeOH (2.0 mL) was stirred at 25° C. for 1 h. Sodium cyanoborohydride (6.9 mg, 110 μmol) was then added, and the resulting mixture was stirred at 25° C. for 16 h. The reaction mixture was then concentrated in vacuo, dissolved in 2 mL DMSO+20 μL trifluoroacetic acid, filtered, and purified by rpHPLC (10-100% acetonitrile/water+0.1% TFA) to provide 1-((2-methyl-4-(5-(1-phenylcyclopropyl)thiazolo[5,4-b]pyridine-2-yl)-phenyl)methyl)-azetidine-3-carboxylic acid as a white solid. MS (ESI) m/z: Calculated: 455.2; Observed: 456.1 (M++1).

1. stirringthe resulting mixture was stirred at 25° C. for 16 h
2. concentrationThe reaction mixture was then concentrated in vacuo
3. dissolutiondissolved in 2 mL DMSO+20 μL trifluoroacetic acid
4. filtrationfiltered
5. custompurified by rpHPLC (10-100% acetonitrile/water+0.1% TFA)