反应详情

Sodium methoxide (0.73 g, 13.5 mmol) was added to 2 methyl 3-[(3-methoxy-3-oxopropanoyl)amino]pyridine-2-carboxylate (0.9 g, 3.4 mmol) dissolved in THF (20 mL). The reaction was stirred overnight. Water was added and the solvents removed. The residue was dissolved in water and purified by reverse phase HPLC eluting with a gradient of 5-40% acetonitrile/water (0.1% TFA) to give the product as a yellow solid.

1. customthe solvents removed
2. dissolutionThe residue was dissolved in water
3. custompurified by reverse phase HPLC
4. washeluting with a gradient of 5-40% acetonitrile/water (0.1% TFA)