Diethylene Glycol Monoethyl Ether → Diethylene Glycol Monoethyl Ether
Reaction equation
[CH3:1][CH2:2][O:3][CH2:4][CH2:5][O:6][CH2:7][CH2:8][OH:9].O=[C:11]1O[C@H:16]([C@H:18]([CH2:20]O)[OH:19])[C:14](O)=[C:12]1O>CS(C)=O>[CH3:1][CH2:2][O:3][CH2:4][CH2:5][O:6][CH2:7][CH2:8][OH:9].[CH:11]1[C:12]([C@H:4]2[CH2:5][O:6][C:7]3[CH:8]=[C:18]([OH:19])[CH:16]=[CH:14][C:12]=3[CH2:11]2)=[CH:14][CH:16]=[C:18]([OH:19])[CH:20]=1Participants
Reactant 2
Product 2
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 37 °C
Procedure
Approximately 20 to 40 mg of the test material was weighed into pre-tared sterile glass vials and the precise weight was recorded. Vehicle volume was then calculated to give a 50% w/v solution, and the vehicle was added. Two different vehicles were used, as indicated in Examples. DMSO vehicle was prepared from 100% DMSO (EMD Biosciences Cat. #MX1458-6, Lot #42364321). Transcutol vehicle was prepared from 100% transcutol (Gattefosse a.s.a., Cedex, France). Samples were then vortexed vigorously until the dry powder was visually brought into solution. In some cases, samples need to be briefly warmed to 37° C. Stock solution aliquots can be frozen in small aliquots and maintained at approximately −20° C., or used immediately after preparation. Racemic mixtures of equol were prepared for testing at concentrations of 0 (control), 0.3, 1.0, and 5.0%. A positive control was prepared using ascorbic acid at a concentration of 50 μg/ml in DMEM/F-12. After stock solutions were diluted for use they were then discarded.
Workup
- customto give a 50% w/v solution
- additionthe vehicle was added
- temperatureStock solution aliquots can be frozen in small aliquots
- temperaturemaintained at approximately −20° C.
- customafter preparation
- customA positive control was prepared
- additionAfter stock solutions were diluted for use they
Sources & evidence
- ORD record
- ord-5df52dd7989a4be2aca6b714e83f96b3
- Source dataset
- uspto-grants-2014_03
- Patent number
- US08668914B2
Reaction notes
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