反应详情

The Example 1 compound, tert-butyl 4-(3-(5-(6-methoxy-1-oxoisoindolin-2-yl)furan-2-carboxamido)pyridin-4-yl)piperazine-1-carboxylate (1.5) (0.1 mmol) was cooled to 0° C. in 1 mL of dioxane and was added to 4N HCl in dioxane (1 mL) and stirred at room temperature for 30 min. Dioxane was removed under vacuo, redissolved in water-acetonitrile and freezed and lyophilized to obtain 5-(6-methoxy-1-oxoisoindolin-2-yl)-N-(4-(piperazin-1-yl)pyridin-3-yl)furan-2-carboxamide (Example 1) as white powder. HPLC-MS tR=0.75 min (UV254 nm); Mass calculated for formula C23H23N5O4, 433.18, observed LCMS m/z 434.20 (M+H)

1. customDioxane was removed under vacuo
2. dissolutionredissolved in water-acetonitrile