反应详情

To 100 ml of a saturated solution of hydrochloric acid in ethanol 5 g of 4-[(S)-4-tert-Butoxycarbonyl-2-({4-[2-((S)-2-cyclobutylcarbamoyl-pyrrolidin-1-yl)-2-oxo-ethoxy]-7-methyl-quinoline-2-carbonyl}-amino)-butyryl]-piperazine-1-carboxylic acid ethyl ester was added and the mixture was stirred for 2 h. Then, 30 ml of toluene was added and the solvents were removed under reduced pressure. The residue was filtered through a plug of silica gel eluting with a gradient of n-heptane/ethyl acetate. The fractions containing the product were combined and the solvent evaporated under reduced pressure. The residue was purified by re-crystallisation from n-heptane/ethyl acetate and dried under reduced pressure. Yield: 3.8 g MS (ES+): m/e=709.

1. customthe solvents were removed under reduced pressure
2. filtrationThe residue was filtered through a plug of silica gel eluting with a gradient of n-heptane/ethyl acetate
3. additionThe fractions containing the product
4. customthe solvent evaporated under reduced pressure
5. customThe residue was purified by re-crystallisation from n-heptane/ethyl acetate
6. customdried under reduced pressure