反应详情

A mixture of (4-{3-[6-(4-chloro-benzylamino)-pyridin-2-yl]-imidazo[1,2-a]pyrazin-8-ylamino}-cyclohexyl)-carbamic acid tert-butyl ester (214 mg, 0.39 mmol) in ethanol (4 mL) and concentrated HCl (4 mL) was stirred at room temperature overnight. The reaction mixture was then concentrated under reduced pressure. The residue was purified by preparative-HPLC to give N-{3-[6-(4-chloro-benzylamino)-pyridin-2-yl]-imidazo[1,2-a]pyrazin-8-yl}-cyclohexane-1,4-diamine; hydrochloride. (Yield 80 mg). 1HNMR (300 MHz, CD3OD): δ 8.22 (d, 1H, J=5.7 Hz), 8.19 (s, 1H), 7.89 (t, 1H, J=8.7 Hz), 7.46-7.39 (m, 4H), 7.23 (d, 1H, J=6.9 Hz), 7.17 (d, 1H, J=5.7 Hz), 7.02 (d, 1H, J=8.7 Hz), 4.70 (s, 2H), 4.04 (brs, 1H), 3.25 (brs, 1H), 2.23 (brs, 4H), 1.81-1.71 (m, 4H). LC-MS: [M+H]+ 448.

1. concentrationThe reaction mixture was then concentrated under reduced pressure
2. customThe residue was purified by preparative-HPLC