反应详情

Commercially available Nα-Fmoc-Nδ-Boc-L-ornithine (1.00 g), WSCI hydrochloride (0.633 g), and HOBt (0.446 g) were reacted in methanol (20 ml) for 23 hours at room temperature. After the reaction, methanol was removed and the residue was extracted with chloroform. The extract was washed with water, saturated aqueous solution of sodium hydrogencarbonate, and saturated brine, and dried over anhydrous sodium sulfate to remove water. Syrup obtained by removing the solvent was purified by silica gel column chromatography (50 g, chloroform/ethyl acetate=5/1) to obtain the title compound (1.06 g) as a white solid.

1. customwas removed
2. extractionthe residue was extracted with chloroform
3. washThe extract was washed with water, saturated aqueous solution of sodium hydrogencarbonate, and saturated brine
4. dry with materialdried over anhydrous sodium sulfate
5. customto remove water
6. customSyrup obtained
7. customby removing the solvent
8. customwas purified by silica gel column chromatography (50 g, chloroform/ethyl acetate=5/1)