Phosphate → Indole

HRID 1198098Notes

Reaction equation

HRID 1198098 reaction equation
P([O-])([O-])([O-])=O.CCC(CO[C:13]([C:26]([N:28](CC[NH+](C)C)C)=O)(C1C=CC=CC=1)[C:14]1[CH:19]=[CH:18][CH:17]=[CH:16][CH:15]=1)CC.[Cl-]>>[NH:28]1[C:19]2[C:14](=[CH:15][CH:16]=[CH:17][CH:18]=2)[CH:13]=[CH:26]1

Participants

3

Reactant 2

HCID 1061PhosphateHCID 19153X 100

Product 1

HCID 798Indole

Procedure

50 mg of wet cells were added to 1 ml of a reaction solution for measurement of the tryoptophanase activity (0.1M phosphate buffer pH 8.0, 10 mM tryptophan, 0.1% Triton X-100), and reacted at 37° C. for 30 minutes. Indole formed was reacted with p-dimethylbenzaldehyde and spectrophotometically quantitated. Some strains exhibit high activity and from these cells a plasmid was extracted by the alkaline SDS method. [T. Maniatis, E. F. Fritsch and J. Sambrook: "Molecular Cloning" (1982) pages 90 to 91] and digested with BamHI and HindIII. Fragment length of the digest was determined by using an agarose gel. It was found that a DNA fragment of about 3.2 kb was inserted into the HindIII and BamHI sites of pBR322 (this plasmid will be referred to as pBR322tna).

Workup

  1. customreacted at 37° C. for 30 minutes

Sources & evidence

ORD record
ord-7f4644a02a35487cb8f2c88ccdb1601c
Source dataset
uspto-grants-1994_01
Patent number
US05279951

Reaction notes

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