Potassium Phosphate, Tribasic → 2-keto-L-Gulonic acid

HRID 1199632Notes

Reaction equation

HRID 1199632 reaction equation
[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([CH:7]=[O:8])=[O:6].P([O-])([O-])([O-])=[O:14].[K+].[K+].[K+]>O>[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([C:7]([OH:14])=[O:8])=[O:6]

Participants

4

Reactant 2

HCID 62657Potassium Phosphate, TribasicHCID 10921067Sorbosone, L-2×

Product 1

HCID 1024242-keto-L-Gulonic acid

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

The reaction mixture containing 100 ml of cell free extract of UV-10, FERM BP-1267 (total enzyme activity, 115 units), as prepared by the manner as described in steps (1) to (2) of Example 1, 50 ml of 0.5M potassium phosphate buffer (pH 7.0), 50 ml of 10% L-sorbosone solution and 300 ml of water was incubated at 30° C. with gentle shaking. As a result, 2-keto-L-gulonic acid was formed with the rate of 700 mg/hr.

Workup

  1. additionThe reaction mixture containing 100 ml of cell
  2. extractionfree extract of UV-10, FERM BP-1267 (total enzyme activity, 115 units)
  3. customas prepared by the manner
  4. customwas incubated at 30° C. with gentle shaking

Sources & evidence

ORD record
ord-4168be1f456842918a9ccfe6e259e046
Source dataset
uspto-grants-1990_02
Patent number
US04902617

Reaction notes

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