Potassium Phosphate, Tribasic → 2-keto-L-Gulonic acid

Participants

Reaction Steps

The reaction mixture containing 100 ml of cell free extract of UV-10, FERM BP-1267 (total enzyme activity, 115 units), as prepared by the manner as described in steps (1) to (2) of Example 1, 50 ml of 0.5M potassium phosphate buffer (pH 7.0), 50 ml of 10% L-sorbosone solution and 300 ml of water was incubated at 30° C. with gentle shaking. As a result, 2-keto-L-gulonic acid was formed with the rate of 700 mg/hr.

Provenance & Evidence