Glycine → L-Aspartic Acid
Reaction equation
[NH2:1][CH2:2][C:3]([OH:5])=[O:4].C1(C[C:13](=O)[C:14]([O-:16])=[O:15])C=CC=CC=1.N[C@H](C(O)=O)CC1C=CC=CC=1>>[NH2:1][C@H:2]([C:3]([OH:5])=[O:4])[CH2:13][C:14]([OH:16])=[O:15]Participants
4
Procedure
Additionally, the strains were inoculated into 100 ml of a similar medium containing glycine instead of the aspartic acid. The cultures were inoculated at 30° C. on a shaking machine for 5 days. Samples were taken after 3 and 5 days. 7 strains from the cultures tested converted phenylpyruvate into phenylalanine in a yield of 30% to 60%, and these were therefore suitable for further adaptation.
Workup
- customafter 3 and 5 days
Sources & evidence
- ORD record
- ord-65ed5fa386214c58ad8c94a06584be95
- Source dataset
- uspto-grants-1988_05
- Patent number
- US04745059
Reaction notes
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