反应详情

Dissolve 2-(4-(2-benzoylsulfanyl-3-phenyl-propionyl-amino)-3-oxo-1,3,4,5-tetrahydro-benzo[c]azepin-2-yl)-4-methyl-valeric acid (0.229 mmol) in degassed methanol (3 mL) and cool in an ice bath. Treat with degassed 1M aqueous lithium hydroxide (1.0 mL) and stir, allowing the ice bath to warm gradually over 3 hours. With the reaction at 0° C., acidify with 5% hydrochloric acid. Partition between methylene chloride (75 mL) and water (25 mL), dry (Na2SO4) and purify by silica gel chromatography (3:1:0.01 hexane/ethyl acetate/acetic acid followed by 1:1:0.01 hexane/ethyl acetate/acetic acid to give the title compound as a white solid (84 mg, 80.8%).

1. temperaturecool in an ice bath
2. additionTreat
3. customwith degassed 1M aqueous lithium hydroxide (1.0 mL)
4. temperatureto warm gradually over 3 hours
5. customWith the reaction at 0° C.
6. customPartition between methylene chloride (75 mL) and water (25 mL)
7. dry with materialdry (Na2SO4)
8. custompurify by silica gel chromatography (3:1:0.01 hexane/ethyl acetate/acetic acid