反应详情

11.83 g of Boc-Phe-Met-OMe (see Biochemistry, 8, 4183 (1969), incorporated herein) is dissolved in 100 ml methylene chloride after which HCl is passed into the solution for about 40 minutes. After evaporation of the solution to dryness, 75 ml ethyl acetate is added, resulting in a precipitate. The solid substance is separated by filtration, washed with petroleum ether, and dried. Melting point 123°-124° C. Rf in To:EtOH (8:2)=0.43 on SiO2.

1. customAfter evaporation of the solution to dryness, 75 ml ethyl acetate
2. additionis added
3. customresulting in a precipitate
4. customThe solid substance is separated by filtration
5. washwashed with petroleum ether
6. customdried