反应详情

A mixture of 4.50 g (15 mM) of 6-[4-(L-alanylamino)-phenyl]-1,2-dihydro-2-oxonicotinic acid, Example 4, 1.97 g (15 mM) N-acetyl-L-alanine, 2.02 g (15 mM) 1-hydroxybenzotriazole, and 150 ml of DMF was stirred at room temperature and 3.09 g (15 mM) of dicyclohexylcarbodiimide was added. After 19 hours the reaction mixture was filtered and the filtrate evaporated to dryness. Water was added and the solids filtered, washed with hot ethyl acetate and ether, and dried, giving 5.01 g of 6-[4-(N-acetyl-L-alanyl-L-alanylamino)phenyl]-1,2-dihydro-2-oxonicotinic acid.

1. filtrationAfter 19 hours the reaction mixture was filtered
2. customthe filtrate evaporated to dryness
3. additionWater was added
4. filtrationthe solids filtered
5. washwashed with hot ethyl acetate and ether
6. customdried