反应详情

温度

37 °C

The enzymatic activity of the acylase in solution, before and after fixing, is determined by adding 0.1 ml. of the enzyme solution to 5 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4 mols of cobalt chloride. The mixture is maintained for 30 minutes at 37° C., whereupon the reaction is stopped by immersing the receptacle in boiling water for 3 minutes. After cooling, the L-methionine formed is determined.

1. additionby adding 0.1 ml
2. customfor 3 minutes
3. temperatureAfter cooling