Sorbitol → L-(-)-Sorbose

Participants

Reaction Steps

A reaction mixture (total volume 1.04 ml) containing 0.2 ml of purified SLDH (0.04 mg protein), 0.04 ml of 0.2M PMS, 0.1 ml of 0.4M D-sorbitol, 0.4 ml of 0.5M potassium phosphate buffer (pH 7.0) and 0.3 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbose was formed at the rate of about 1.3 mg/hour.

Provenance & Evidence