Sorbitol → L-(-)-Sorbose

HRID 1288658Notes

Reaction equation

HRID 1288658 reaction equation
[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+]>O>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

4

Reactant 2

HCID 5780SorbitolHCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 6904L-(-)-Sorbose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

A reaction mixture (total volume 1.04 ml) containing 0.2 ml of purified SLDH (0.04 mg protein), 0.04 ml of 0.2M PMS, 0.1 ml of 0.4M D-sorbitol, 0.4 ml of 0.5M potassium phosphate buffer (pH 7.0) and 0.3 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbose was formed at the rate of about 1.3 mg/hour.

Workup

  1. customwas incubated at 30° C. with gentle shaking

Sources & evidence

ORD record
ord-a3562e6deb6a4016935eaf47602ff2ab
Source dataset
uspto-grants-1998_05
Patent number
US05747301

Reaction notes

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