反应详情

温度

2 °C

To a cold suspension of 75.3 g (191 mmol) (3β)-cholesta-5,7,16-trien-23-yne-3,25-diol in 450 mL of dichloromethane was added 107 mL (1.14 mol) acetic anhydride and 160 mL (1.14 mol) triethylamine. After the suspension was cooled to 2° C., 4.6 g (38 mmol) 4-dimethylaminopyridine was added. After 10 min, the cold bath was removed and the mixture was stirred at room temperature overnight. After cooling again with an ice-water bath, 30.7 mL (758 mmol) methanol was added, and the mixture was stirred at room temperature for 1.5 hr. The mixture was washed with water and the aqueous layer was extracted with dicholormethane. The combined organic layers were washed with 200 mL 1N HCl, and the aqueous layer was extracted with dichloromethane. The combined organic layers were washed with saturated aqueous NaHCO3, dried over Na2SO4, and concentrated to dryness. The residue was dissolved in 700 mL of warm 95% methanol. After a seed crystal was added, the suspension was stored in a refrigerator overnight. The precipitate was filtered and washed with 700 mL of cold 90% methanol. After being dried under high vacuum, 82.05 g (89.8%) of 3β)-cholesta-5,7,16-trien-23-yne-3,25-diol diacetate was obtained as a white solid: mp 98°-101° C. Anal Calcd for C31 H42O4 : C, 77.79; H, 8.94. Found C, 77.74; H, 8.88.

1. customthe cold bath was removed
2. temperatureAfter cooling again with an ice-water bath
3. stirringthe mixture was stirred at room temperature for 1.5 hr
4. washThe mixture was washed with water
5. extractionthe aqueous layer was extracted with dicholormethane
6. washThe combined organic layers were washed with 200 mL 1N HCl
7. extractionthe aqueous layer was extracted with dichloromethane
8. washThe combined organic layers were washed with saturated aqueous NaHCO3
9. dry with materialdried over Na2SO4
10. concentrationconcentrated to dryness
11. dissolutionThe residue was dissolved in 700 mL of warm 95% methanol
12. additionAfter a seed crystal was added
13. customwas stored in a refrigerator overnight
14. filtrationThe precipitate was filtered
15. washwashed with 700 mL of cold 90% methanol
16. dry with materialAfter being dried under high vacuum, 82.05 g (89.8%) of 3β)-cholesta-5,7,16-trien-23-yne-3,25-diol diacetate
17. customwas obtained as a white solid