Udp-Glucose → Sucrose

Participants

Reaction Steps

It was found that the hitherto used hepes-NaOH-buffer was most suitable for the synthesis of UDP-glucose and TDP-glucose. For both enzymes, the Mops-buffer and the TES-buffer gave 60 to 80% residual activity. The commercial enzyme indicated, by contrast to the rice enzyme, in TEA-buffer, an about 30 to 40% higher residual activity. In the remaining buffers, both enzymes had a residual activity of less than 50%.

Provenance & Evidence