HRID1289357

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=c1ccn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID8629

Uridine diphosphate glucose

C15H24N2O17P2

结构：Cc1cn([C@H]2C[C@H](O)[C@@H](COP(=O)(O)OP(=O)(O)OC3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)O2)c(=O)[nH]c1=O

HCID439324

未命名化合物

结构：O=S(=O)(O)CCN1CCN(CCO)CC1.[Na+].[OH-]

HCID86606154

未命名化合物

PRODUCTS

生成物

结构：OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O

HCID5988

Sucrose

C12H22O11

PROCEDURE

实验过程

It was found that the hitherto used hepes-NaOH-buffer was most suitable for the synthesis of UDP-glucose and TDP-glucose. For both enzymes, the Mops-buffer and the TES-buffer gave 60 to 80% residual activity. The commercial enzyme indicated, by contrast to the rice enzyme, in TEA-buffer, an about 30 to 40% higher residual activity. In the remaining buffers, both enzymes had a residual activity of less than 50%.

WORKUP

后处理

customFor both enzymes, the Mops-buffer and the TES-buffer gave 60 to 80% residual activity