Hydrochloric Acid → L-Tryptophan

Participants

Reaction Steps

Placed in a 100-ml Erlenmeyer flask were 19 ml of one of the L-serine solutions and 0.2 ml of one of the PLP solution. Thiophenol (0.5 ml) was added, followed by incubation at 35° C. for 5 minutes. The cell suspension (0.5 ml) was added, followed by incubation for 60 minutes under shaking. Sixty minutes later, 1 ml of 35% hydrochloric acid was added to terminate the reaction, and the concentration of S-phenyl-L-cysteine in the solution was quantitated by HPLC. The amount (g) of S-phenyl-L-cysteine formed in an hour per gram of the dried cells was defined as S-phenyl-L-cysteine forming activity.

Provenance & Evidence