反应详情

Reactions were assembled in a volume of 260 μl containing 50 mM Hoes buffer, 150 mM NaCl, pH 7.5 and as indicated 5 μM SrtA-DN in 50 mM BisTris, pH 7.5, 10 AM LPXTG (SEQ ID NO: 1) peptide (DABCYL-QALPETGEE-EDANS) (QALPETGEE is SEQ. ID NO: 39), 10 μM TGXLP (SEQ ID NO 45) peptide (DABCYL-QATGELPEE-EDANS) (QATGELPEE is SEQ ID NO: 46), 5 mM MTSET, 0.2 M NH-2-OH, 5 mM pHMB or 10 mM DTT. Incubations were carried out for 1 hour at 37° C. Samples were analyzed in a fluorimeter using 395 nm for excitation and 495 nm for recordings.

1. additioncontaining 50 mM Hoes buffer